Melanin concentrating hormone (MCH) and Gonadotrophin releasing hormone (GnRH) as regulators of somatolactin function in the teleost fish Cichlasoma dimerus.

CÁNEPA, MAXIMILIANO; POZZI, ANDREA; ASTOLA, ANTONIO; MAGGESE, M. CRISTINA; VISSIO, PAULA G.

CELL AND TISSUE RESEARCH

Springer-Verlag

Lugar: Berlin; Año: 2008 vol. 333 p. 49 - 59

0302-766X

We detected a close morphological associationbetween melanin-concentrating hormone (MCH)-immunoreactive (ir) fibers and somatolactin(SL)-ir cells in the pars intermedia of the cichlid fishCichlasoma dimerus by double-labelimmunofluorescence. Male pituitaries obtained from adult C.dimerus were incubated with 0.1?10 µM salmonMCH,and the amount of SL released into the culturemedium was semi-quantified by Western blot. Thisassay showed an increase of SL release in adose-dependent manner (linear regression:P