CHARACTERIZATION OF AN ACTIVE FIBROSA FILM WITH MCCJ25 (G12Y) / TWEEN 80

KOLTAN, MARIANO; RUTH ARANCELI PONCE; MARIANA BLANCO MASSAN; POMARES, MARÍA FERNANDA; PATRICIA EISENBERG; VINCENT PAULA A.

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

In Argentina, the hemolytic uremic syndrome (HUS) is considered endemic, and the principal infective agent is Escherichia coliO157:H7. HUS is the main cause of acute renal failure and thesecond cause of chronic kidney failure and kidney transplantationin children in Argentina. In the meat industry, several episodes ofcontamination in fermented dry sausages were registered with thispathogen. Active packaging technology employing polymeric materials as support for antimicrobial agents can improve the food hygienic-sanitary quality. The Fibrous film is used for embedding salamis.the obtaining of an active film would be of great importance in theproduction of artisan salamis since there is no rigorous control of thepH and water activity, which, can lead to a slow acidification, wherethe growth of pathogenic microorganisms could be favored. MicrocinJ25(G12Y) [MccJ25 (G12Y)] is an antimicrobial peptide with greatpotential as an additive in the food industry for its activity on E. coliO157: H7 and its sensitivity to digestive tract enzymes. Our resultsdemonstrated that MccJ25(G12Y) did not inhibit the growth of E.coli O157: H7 in meat, at least up to 4 mg/ml of microcin concentration. The hypothesis was that the elevated fat content of the meatcould be interfering in microcin activity. Tween 80 is a surfactantapproved by the United States Food and Drug Administration (FDA)for use in foods, which has the ability to destabilize and emulsifyfats. Surprisingly, our results showed that the addition of MccJ25(-G12Y) (4mg/ml) + Tween 80 (0.1%) totally inhibited the growth of E.coli O157: H7 in meat. According to this, the objective of this workwas the characterization of a fibrous film active with MccJ25(G12Y)+Tween 80 for its potential use in the meat industry. Based on this,the aims were determined the minimum contact time, minimum MccJ25(G12Y) concentration for film activation, stability of active filmand microcin migration in food simulants.Keywords: Es