Pseudomonas putida like bioinoculant on soybean and corn irrigated with vinasse

LAMI, MARÍA JESÚS; COSTA GUTIERREZ, SB; MICHAVILA GABRIELA; CARAM DI SANTO, CAROLINA; ANA ZENOFF; ESPINOSA URGEL, M; VINCENT, PAULA A.; DE CRISTÓBAL, RICARDO

Congreso; L Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología molecular; 2014

The vinasse is the residue of ethanol production, which is the largest source of water pollution from the Sali-Dulce Basin. Actually it is used to irrigate crops (fetiriego). Recent work in Pseudomonas putida KT2440 shows that the role of matrix components LapA y LapF which are the two major secreted proteins are involved in the development of biofilms. This study showed that mutants defective in these proteins have increased production of exospolisacarido (compensatory mechanism) compared to the wild type, giving greater resistance to various stressors. In this work the influence of KT2440 and mutant were studied as bioinoculants in corn and soybean plants treated with vinasse. First, we tested the influence of irrigation with pure and diluted vinasse in the growth of these crops. Then we determine the MIC of vinasse and growth in mixed media where LB / M9-vinasse was obtained in both cases that shows a better growth of KT2440 and mutant LapF. We performed in vitro and in vivo with corn and soybeans irrigated with vinasse where bioinoculated with the above strains. A better germination and plant growth were shown in both cases. These results suggest that the use of P. putida as bioinoculant in soybean and corn generates the vinasse plant tolerance.