Metabolic profiling analysis of potato with altered expression of the antimicrobial gene snakin-1.

NAHIRÑAK, V; ALMASIA, NI; HOPP, EH; CARRARI F; VAZQUEZ ROVERE, C

Tucuman

Congreso; XLV reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009

SAIB

Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum that was shown to be active against pathogens both in vitro and in vivo. We have previously obtained and characterized potato transgenic lines overexpressing or silencing StSN1 gene. Based on the enhanced resistance of the overexpressing lines to different pathogens, we decided to explore the metabolic changes undergoing in lines with altered expression of StSN1. To this end, we carried out metabolic profiling on potato leaves using a gas chromatography?mass spectrometry approach. For this purpose, we chose three transgenic lines with higher expression levels of StSN1 than wild type genotype, and other five with repressed levels. Using data-mining tools, the metabolic profiles from these experiments were analyzed to determine possible clusters. Integration of these data with previous results from transcriptional profiling analysis and phenotypic characterization of these lines, will allow us to deep into the knowledge of the in-vivo role of this gene.