Central natriuretic peptides regulation of peripheral atrial natriuretic factor release

PUYO AM; VATTA MS; DONOSO AS; BIANCIOTTI LG; FERNADEZ BE

REGULATORY PEPTIDES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2000 vol. 90 p. 93 - 99

0167-0115

Atrial natriuretic factor (ANF) and C-type natriuretic peptide (CNP) receptors have been described in encephalic areas and nucleirelated to the regulation of cardiovascular as well as sodium and water homeostasis. Stimulation of the anterior ventral third ventricularregion of the brain modifies plasma ANF concentration, suggesting the participation of the central nervous system in the regulation ofcirculating ANF. The aim of this work was to study the effect of centrally applied ANF or CNP on plasma ANF. Normal and bloodvolume expanded rats (0.8 ml isotonic saline /100 g body weight) were intra cerebralventricularly injected with 1, 10 or 100 ng/ml /minANF. Blood volume expanded animals were also centrally injected with the same doses of CNP. Blood samples were collected at 5 and 15min. after intracerebralventricular administration of either ANF or CNP. Centrally applied ANF did not affect circulating ANF in normalblood volume rats. In blood volume expanded animals both ANF (1, 10 or 100 ng/ml /min) and CNP (1 ng/ml /min) decreased plasmaANF concentration after 15 min. Moreover, CNP (10 and 100 ng/ml /min) lowered circulating ANF levels not only at 15 min but also at 5min. Neither ANF nor CNP elicited any change in mean arterial pressure and heart rate in normal and blood volume expanded rats. Theseresults suggest the existence of a central regulation exerted by natriuretic peptides on circulating ANF levels. Furthermore, this is the firststudy reporting an effect on plasma ANF induced by centrally applied CNP.