INVESTIGADORES
URTREGER Alejandro Jorge
congresos y reuniones científicas
Título:
Role of retinoic acid receptors a and g on cell growth and stem cell maintenance in breast cancer
Autor/es:
CAROLINA FLUMIAN; DAMIAN E. BERARDI; ALEJANDRO J. URTREGER; LAURA B. TODARO
Lugar:
Buenos Aires
Reunión:
Congreso; XLIX Reunion Anual Sociedad Argentina de Investigacion en Bioquimica y Biologia Molecular; 2013
Institución organizadora:
Sociedad Argentina de Investigacion en Bioquimica y Biologia Molecular
Resumen:
Due to their role in the regulation of cell growth and differentiation, retinoids (Rds) are being evaluated in clinical trials for cancer prevention and treatment. Rds exert their functions through the binding to the nuclear receptors RAR and RXR. We focus our studies in LM38-LP, a murine mammary tumor cell line composed by luminal (LEP), myoepithelial (MEP) and stem/progenitor cells (MSC) that expresses all functional retinoic acid receptors. Our objective was to evaluate the effect of RARa and RARg activation on cell growth, alteration of LEP/MEP proportion and their role on MSC growth and maintenance. AM580 (RARa agonist) and AM580+MM (RARg antagonist), diminished cell proliferation after 144 H treatment. Whereas MM, Ro41 (RARa antagonist), BMS (RARg agonist) and their combinations had no effect on proliferative potential. Through flow cytometry, we could determine that all treatments decreased LEP/MEP ratio (CD24+CD29+/CD24-CD29+). Regarding MSC, through primary mammosphere formation assays, we observed that AM580, MM, AM580+MM and BMS+Ro41 treatments increased mamosphere number. But, while BMS, Ro41 and BMS+Ro41 increased mamosphere size, AM580 induced the opposite effect. In sum, both RARa and g decrease the proportion of LEP vs. MEP cells but only RARa induces proliferation inhibition. Besides, RARa would participate in MSC survival while RARg would be involved in MSC growth.