Modulation of fibronectin expression and proteolytic activity associated with the invasive and metastatic phenotype in two new murine mammary tumor cell lines

ALEJANDRO J. URTREGER; VIRGINIA LADEDA; MARÍA DEL CARMEN VIDAL; LYDIA I. PURICELLI; EUGENIA SACERDOTE DE LUSTIG; ELISA D. BAL DE KIER JOFFE

INTERNATIONAL JOURNAL OF ONCOLOGY

SPANDIDOS PUBL LTD

Año: 1997 vol. 11 p. 489 - 496

1019-6439

Two cell lines obtained by sequential subcultures from primary cultures of the murine mammary adenocarcinomas M3 and MM3 were characterized. During LM3 cell line evolution an increase in the anchorage-independent growth ability, a displacement towards a triploid level in the chromosomic distribution and a loss of fibronectin (FN) expression were observed. LMM3 cell line showed a tetraploid chromosomic number and was unable to produce FN, behaving in a similar way as its parental tumor MM3. The local invasive ability as well as the spontaneous metastatic capacity of LM3 cells inoculated s.c. increased with the passage number and were associated to the progressive loss of FN expression as well as to the appearence of membran bound uPA. LMM3 cells, which never expressed FN, maintained the high spontaneous metastasic capacity already shown by the parental tumor MM3 but acquired a higher local invasive phenotype.