INVESTIGADORES
URTREGER Alejandro Jorge
artículos
Título:
Opposite effects of protein kinase C beta1 (PKCb1) and PKCe in the metastatic potential of a breast cancer murine model
Autor/es:
VALERIA C. GROSSONI; LAURA B. TODARO; MARCELO G. KAZANIETZ; ELISA D. BAL DE KIER JOFFÉ; ALEJANDRO J. URTREGER
Revista:
BREAST CANCER RESEARCH AND TREATMENT
Editorial:
Springer Netherlands
Referencias:
Lugar: Dordrecht; Año: 2009 vol. 118 p. 469 - 480
ISSN:
0167-6806
Resumen:
In this paper we investigated whether protein kinase C (PKC) b and PKCe, members of the classical and novel PKC family respectively, induce phenotypic alterations that could be associated with tumor progression and metastatic dissemination in a murine model of breast cancer. Stable overexpression of PKCb in LM3 cells altered their ability to proliferate, adhere, and survive, and impaired their tumorigenicity and metastatic capacity. Moreover, PKCb induced the re-expression of fibronectin, an extracellular matrix glycoprotein which loss has been associated with the acquisition of a transformed phenotype in different cell models, and exerted an important inhibition on proteases production, effects that probably impact on LM3 invasiveness and dissemination. Conversely, PKCe overexpression enhanced LM3 survival, anchorage-independent growth, and caused a significant increase in spontaneous lung metastasis. Our results suggest PKCb functions as an inhibitory protein for tumor growth and metastasis dissemination whereas PKCe drives metastatic dissemination without affecting primary tumor growth.