POPULATION GENETIC STUDY OF Gibberella zeae (Fusariumgraminearum) ISOLATED FROM WHEAT IN URUGUAY

PAN, DINORAH; REYNOSO M.M.; TORRES, A.M.

Mérida

Congreso; VI Latin American Congress of Mycotoxicology and II International Symposium on algal and fungal toxins for Industry; 2010

Sociedad Latinoamericana de Micotoxicologia

In Uruguay, F. graminearum is the most common species that infects wheat and is responsible for Fusarium head blight and contamination of grain with deoxynivalenol (DON). The development of measures to control this disease makes it necessary to know the population genetic structure of F. graminearum to understand the epidemiology and evolutionary potential and to improve strategies to control this fungal pathogen. The aim of this study was to determine the genetic structure of populations of F. graminearum isolated from wheat in Uruguay. A total of 151 AFLP bands were identifided in the 200-600 pb range from the 61 analysed isolates when using the three primer pair combinations. The analysis of profiles generated by AFLP revealed the presence of 151 loci from the 61 strains. The primer combination EcoRI + CC and MseI + CG amplified bands of 45, while the combination EcoRI and MseI + TG + TT and EcoRI + AA and MseI + AT amplified bands 79 and 27, respectively. The genetic similarity between isolates of F. graminearum analyzed was 72.5% (range = 47.7 - 93.5%). The genetic similarity coefficients indicate that any of the isolate belonging to the same species are greater than 0.60 (60%). In the present study we included strains identified as belonging to F.graminearum lineages 1-7 in order to obtain the standard AFLP profiles for subsequent comparison with our strains. All isolates analyses had AFLP profiles typical of F. graminearum lineage 7. Knowing the lineage of F. graminearum that prevalent in Uruguay provides information on the origin of our population, as well as the potential route of entry of this pathogen. This knowledge could prevent the inadvertent introduction of foreign populations.F. graminearum is the most common species that infects wheat and is responsible for Fusarium head blight and contamination of grain with deoxynivalenol (DON). The development of measures to control this disease makes it necessary to know the population genetic structure of F. graminearum to understand the epidemiology and evolutionary potential and to improve strategies to control this fungal pathogen. The aim of this study was to determine the genetic structure of populations of F. graminearum isolated from wheat in Uruguay. A total of 151 AFLP bands were identifided in the 200-600 pb range from the 61 analysed isolates when using the three primer pair combinations. The analysis of profiles generated by AFLP revealed the presence of 151 loci from the 61 strains. The primer combination EcoRI + CC and MseI + CG amplified bands of 45, while the combination EcoRI and MseI + TG + TT and EcoRI + AA and MseI + AT amplified bands 79 and 27, respectively. The genetic similarity between isolates of F. graminearum analyzed was 72.5% (range = 47.7 - 93.5%). The genetic similarity coefficients indicate that any of the isolate belonging to the same species are greater than 0.60 (60%). In the present study we included strains identified as belonging to F.graminearum lineages 1-7 in order to obtain the standard AFLP profiles for subsequent comparison with our strains. All isolates analyses had AFLP profiles typical of F. graminearum lineage 7. Knowing the lineage of F. graminearum that prevalent in Uruguay provides information on the origin of our population, as well as the potential route of entry of this pathogen. This knowledge could prevent the inadvertent introduction of foreign populations.