INVESTIGADORES
STRUMIA Miriam Cristina
artículos
Título:
Preparation of macroporous monoliths based on epoxy-bearing hydrophilic
Autor/es:
DARIO ARRUA; CRISTIAN MOYA; EUGENIA BERNARDI; JORGE ZARZUR; MIRIAM STRUMIA; CECILIA ALVAREZ I.
Revista:
EUROPEAN POLYMER JOURNAL
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2010 p. 663 - 672
ISSN:
0014-3057
Resumen:
This work reports the synthesis and characterization of poly(N-acryloyl-tris(hydroxymethyl)
aminomethane-co-glycidyl methacrylate-co-N,N0-methylenebisacrylamide) [poly-
(NAT-GMA-BIS)] discs using different porogenic solvents. The macroporous polymer
obtained with PEG 6000 as co-porogen showed the best porous properties of the series.
End-point immobilization of heparin (Hep, as ligand) was then carried out on the products
through reductive amination. Thus, the products were reacted with ethylenediamine (EDA)
and hexamethylenediamine (HMDA) in order to introduce amine groups and to analyze the
length of the spacer on the immobilization of heparin. Then, the highest value of amine
groups [1.280 and 0.919 mmol amine/g dry support for poly(NAT-GMA-BIS)-EDA and
poly(NAT-GMA-BIS)-HMDA, respectively] was obtained at 40 C after 48 h of reaction.
Finally, the amount of ligand coupled to discs was not influenced by the two length spacers
assayed. The amount of Hep coupled on discs [591.50 and 489.90 lg Hep/g dry polymer for
poly(NAT-GMA-BIS)-EDA-Hep and poly(NAT-GMA-BIS)-HMDA-Hep, respectively] was
similar to that obtained using macroporous polymer beads reported by other authors.
The supports yielded were used to assay the retention of antithrombin III (AT-III). The
retention was greater for the EDA-containing product with a larger amount of heparin.
These novel heparin-containing macroporous poly(NAT-GMA-BIS) discs could be used as
potential affinity chromatography supports.N-acryloyl-tris(hydroxymethyl)
aminomethane-co-glycidyl methacrylate-co-N,N0-methylenebisacrylamide) [poly-
(NAT-GMA-BIS)] discs using different porogenic solvents. The macroporous polymer
obtained with PEG 6000 as co-porogen showed the best porous properties of the series.
End-point immobilization of heparin (Hep, as ligand) was then carried out on the products
through reductive amination. Thus, the products were reacted with ethylenediamine (EDA)
and hexamethylenediamine (HMDA) in order to introduce amine groups and to analyze the
length of the spacer on the immobilization of heparin. Then, the highest value of amine
groups [1.280 and 0.919 mmol amine/g dry support for poly(NAT-GMA-BIS)-EDA and
poly(NAT-GMA-BIS)-HMDA, respectively] was obtained at 40 C after 48 h of reaction.
Finally, the amount of ligand coupled to discs was not influenced by the two length spacers
assayed. The amount of Hep coupled on discs [591.50 and 489.90 lg Hep/g dry polymer for
poly(NAT-GMA-BIS)-EDA-Hep and poly(NAT-GMA-BIS)-HMDA-Hep, respectively] was
similar to that obtained using macroporous polymer beads reported by other authors.
The supports yielded were used to assay the retention of antithrombin III (AT-III). The
retention was greater for the EDA-containing product with a larger amount of heparin.
These novel heparin-containing macroporous poly(NAT-GMA-BIS) discs could be used as
potential affinity chromatography supports.N,N0-methylenebisacrylamide) [poly-
(NAT-GMA-BIS)] discs using different porogenic solvents. The macroporous polymer
obtained with PEG 6000 as co-porogen showed the best porous properties of the series.
End-point immobilization of heparin (Hep, as ligand) was then carried out on the products
through reductive amination. Thus, the products were reacted with ethylenediamine (EDA)
and hexamethylenediamine (HMDA) in order to introduce amine groups and to analyze the
length of the spacer on the immobilization of heparin. Then, the highest value of amine
groups [1.280 and 0.919 mmol amine/g dry support for poly(NAT-GMA-BIS)-EDA and
poly(NAT-GMA-BIS)-HMDA, respectively] was obtained at 40 C after 48 h of reaction.
Finally, the amount of ligand coupled to discs was not influenced by the two length spacers
assayed. The amount of Hep coupled on discs [591.50 and 489.90 lg Hep/g dry polymer for
poly(NAT-GMA-BIS)-EDA-Hep and poly(NAT-GMA-BIS)-HMDA-Hep, respectively] was
similar to that obtained using macroporous polymer beads reported by other authors.
The supports yielded were used to assay the retention of antithrombin III (AT-III). The
retention was greater for the EDA-containing product with a larger amount of heparin.
These novel heparin-containing macroporous poly(NAT-GMA-BIS) discs could be used as
potential affinity chromatography supports.lg Hep/g dry polymer for
poly(NAT-GMA-BIS)-EDA-Hep and poly(NAT-GMA-BIS)-HMDA-Hep, respectively] was
similar to that obtained using macroporous polymer beads reported by other authors.
The supports yielded were used to assay the retention of antithrombin III (AT-III). The
retention was greater for the EDA-containing product with a larger amount of heparin.
These novel heparin-containing macroporous poly(NAT-GMA-BIS) discs could be used as
potential affinity chromatography supports.