Candida albicans MODULATES ANTIMICROBIAL PEPTIDES OF −DEFENSINS FAMILY DURING VAGINAL INFECTIONS

ANGIOLINI SC; FIGUEREDO CM, VIGEZZI C, MAYOL G, PERALTA-RAMOS JM, MIRÓ MS, RODRIGUEZ E, ICELY PA, SOTOMAYOR CE.; MIRÓ MS; ICELY PA; SOTOMAYOR CE

Córdoba

Congreso; Reunión Conjunta se Sociedades de Biociencias. SAIC, SAI, SAFE, NANOMED; 2021

Sociedades de Biociencias. SAIC, SAI, SAFE, NANOMED

Vulvovaginal Candidiasis (VVC) is an acute inflammatory disease caused by Candida species that affects up to 75% of women of childbearing age once in their life. β-defensins (BDs) are one of the most important families of antimicrobial peptides in the female genital tract with relevant local functions such as antimicrobial and chemoattractant of PMNs, but its role during VVC is limited. Our aim was to study the role of BD1 (constitutive) and BD3 (inducible) in the pathogenesis of VVC using a murine model. Females (8-10-week-old C57BL/6J) were treated subcutaneously with β-estradiol-17?valerate in sesame oil (0.2mg/100μl) on days (D) -6,-3,2 and 4 post infection(pi). On D0 mice were inoculated intravaginally with C. albicans SC5314 suspension (5.106 yeasts/PBS)(infected group), or uninfected (No-infected group). Animals without any treatment were used as controls (untreated). Cervicovaginal lavages (VL) were obtained for the study of different parameters. Infection was evaluated by local fungal load (CFU) and PMNs recruited to vaginal lumen on D2,4 and 8 pi. Fungal burden remained stable from D2 to D4, with a significant decrease at D8(p