Study of virulence factor of Candida species in oral lesions and its association with potentially malignant and malignant lesions

CASTILLO G; LOPEZ DE BLANC S; SOTOMAYOR CE; AZCURRA AI

ARCHIVES OF ORAL BIOLOGY

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2018

0003-9969

Objective: The aim of this study was to explore the association between malignant andpremalignant lesions and the virulence factor profile of Candida spp. recovered fromdifferent oral lesions.Design: Candida spp. isolated from malignant lesions (squamous cell carcinoma, OC, n =25), atypical lichen planus (AL, n = 11), chronic candidiasis (CC, n = 25), andasymptomatic carriers (WI, n = 15, control strains.) Isolates were identified inchromogenic medium, colony morphology and biochemical tests. The lipolytic andproteinase activity was determined on supplemented agar with olive oil and BSA,respectively. The biofilm formation with XTT reduction assay and cellular surfacehydrophobicity (CSH) by water- hydrocarbon method were performed.Results: All isolates recovered from oral lesions produced the four virulence factors studiedwith significantly higher levels than in WI isolates. Interestingly, lipolytic activity wasabsent in WI isolates. The proteolytic activity was similar in AL and OC isolates. OCisolates showed significantly higher CSH values than other clinical isolates. Non-albicans species showed higher biofilm formation than C.albicans (P = 0.03.) There were no significant differences in virulence factors among species. A strong positive correlation was found between proteinase and lipase activity (r = 0.90, P < 0.0001), and between hydrophobicity and biofilm (R = 0.81, P < 0.0001.)Conclusions: Our results indicate that OC Candida isolates exhibited a significant higher attributes of virulence than other lesions fungus isolates, providing evidence about the association between Candida pathogenicity and lesions severity.