Candida albicans Activation of Human Monocytes Toward M2 Profile is Reversed by Amphotericin B and Fluconazole

CAEIRO JP, ROMERO F, CARRIZO S, SPESSO F, RAVERA R, PENCO S, BOISSEAU C, ABIEGA C, SOTOMAYOR CE, ICELY PA, RIERA F; VIGEZZI, CECILIA; RODRIGUEZ EMILSE; MIRÓ MARIA SOLEDAD; SALIDO- RENTARÍA B; SOTOMAYOR, CLAUDIA ELENA

Journal of Bacteriology and Mycology

Austin Publishing Group

Lugar: Texas; Año: 2021 vol. 8 p. 1168 - 1174

2471-0172

Phagocytes, including monocytes/macrophages, play an important role in thehost defense during Candida albicans infections. In the L-arginine metabolism,the balance between the activation of two enzymes, inducible Nitric OxideSynthase (iNOS) and arginase, promotes in the macrophages two alternativemetabolic states, while M1 profile is related with host protection, M2 favored thefungal growth and evasion. Our aim was to evaluate the effect of AmphotericinB (AMB) and Fluconazole (FLC) on polarization of human monocytes to M2profile induced by C. albicans. The human monocytic (Mo) cell line U937 wasco-cultured with viable yeast of C. albicans, or Lipopolysaccharides (LPS) orPhorbol-12-myristate-13-acetate (PMA). Nitric Oxide (NO), cytokines productionand arginase activity were evaluated. The effect of AMB or FLC on thesemetabolic pathways in immune cells and on fungus intrinsic arginase activitywas studied. C. albicans inhibits NO production in human-monocyte and inducesstrong host arginase activity (p