SORDELLI Daniel Oscar
Molecular fingerprinting of Staphylococcus aureus isolated from patients with osteomyelitis in Argentina and clonal distribution of the cap5(8) Genes and of other selected virulence genes.
LATTAR, SANTIAGO M.; TUCHSCHERR LORENA P.N.; CENTRON D; BECKER K; PREDARI S; BUZZOLA FERNANDA R.; ROBINSON D.A.; SORDELLI, DANIEL O.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES : OFFICIAL PUBLICATION OF THE EUROPEAN SOCIETY OF CLINICAL MICROBIOLOGY.
Lugar: Berlin; Año: 2012 vol. 31 p. 2559 - 2566
Abstract. The molecular fingerprinting of a collection of 99 S. aureus isolates from patients with osteomyelitis in Argentina was performed. Twenty-four SmaI PFGE types and 37 spa types were identified. The isolates were assigned to 22 STs. The proportion of MRSA isolates was significantly higher among cap5 S. aureus (40/66) compared with cap8 S. aureus (7/33) isolates (p=0.0003). Twenty-four of the 99 isolates carried the lukS-PV/lukF-PV genes, which were significantly associated to cap5 [(23/66) compared with cap8 S. aureus isolates (1/33) (p=0.0010)]. Forty-five of the 99 isolates carried the seg and sei genes. The distribution of seg/sei genes among isolates was related to certain clones. Isolates of the 4 agr types were found in the S. aureus collection. Whereas agr I isolates were evenly distributed among cap5 and cap8 S. aureus isolates (33/66 and 13/33, respectively), the agr II group was composed of 33 cap5 S. aureus isolates and agr III was composed of 17 cap8 S. aureus isolates. Two clones originally associated to animals (ST 188, 7 isolates and ST 1796, 5 isolates) were associated with chronic osteomyelitis and lack of CP expression. Loss of CP expression remains the single factor among those investigated associated with chronic osteomyelitis.