Quantification of plasma reduced glutathione and liver in cats

DENZOIN, L. SORACI, A.L.; TAPIA. O.

Buenos Aires

Congreso; XL Reunion anual de la Asociación Argentina de Farmacología Experimental SAFE; 2008

SAFE

Glutathione is an important intracellular tripeptide with multiple functions.Abnormal glutathione metabolism is thought to play an important role invarious diseases of cats. However, no data regarding concentration of plasmaglutathione are available for domestic cats. This study discusses thedevelopment of a rapid, simple high pressure liquid chromatography methodfor measurement of reduced glutathione (GSH), oxidized glutathione (GSSH)and total glutathione (GSHt) in plasma, for the purpose of establishing baselinedata for future studies. The mean concentrations of GSH, GSSH and GSHt were4.51  and 0.38 mM ; 19.44 and 3.79 mM (expressed as GSH equivalent) and23.59 and 3.89 mM, respectively. This is the first report of plasma glutathione concentrations in this species