LONG-TERM OVARIAN HORMONE DEPRIVATION INDUCES MITOCHONDRIAL DYSFUNCTION AND CHANGES IN MITOCHONDRIAL MEMBRANE LIPID PROFILE IN THE HIPPOCAMPUS

S.ZÁRATE; M.ASTIZ; N.MAGNANI; M.IMSEN; F.MERINO; S.ALVAREZ; A.REINES; A. SEILICOVICH

Congreso; IBRO 2015; 2015

Mitochondrial dysfunction is involved in age-related disorders. During reproductive senescence, there is a gradual loss of ovarianhormones, w hich exert neuroprotective actions increasing mitochondrial respiratory efficiency and functions. Also, mitochondria functionalintegrity is highly dependent on membrane fatty acid and phospholipid composition, mainly cardiolipin content and its acyl chain composition,parameters that are also affected during aging.The aim of the present w ork w as to study the effect of long-term ovarian hormone deprivation upon mitochondrial function and its putativecorrelation w ith changes in mitochondrial membrane lipid profile in the hippocampus, one of the areas primarily affected in age-relateddisorders and highly responsive to ovarian hormones.To this aim, Wistar adult female rats w ere ovariectomized or sham-operated and after 12 w eeks w e evaluated different parameters ofmitochondrial function as w ell as membrane fatty acid composition and phospholipid content in mitochondria isolated from the hippoccampus.Mitochondria from OVX rats show ed, compared w ith SHAM rats, a marked decrease in the rates of active (state 3) respiration (SHAM: 68 ±4, OVX: 58 ± 6 ng-at O/min.mg protein; p