Estrogens promote GH3 somatolactotrope cell survival through nuclear factor kappa (NF-kB) pathway

G. EIJO; M.A.MORENO AYALA; G. JAITA; S. ZARATE; M.L.MAGRI; M. CANDOLFI; M.F.GOTTARDO; J. FERRARIS; D.PISERA; A. SEILICOVICH

San Francisco

Congreso; 95º Annual Meeting of the Endocrine Society; 2013

Activation of Nuclear Factor kappa B (NF-κB) promotes cell proliferation and inhibits apoptosis. In several cell lines, including GH3 pituitary cells, NF-κB activity is constitutive and sustained. We have previously shown that estrogens inhibit TNF-α- induced NF-κB nuclear translocation in anterior pituitary cells from primary cultures, suggesting that this mechanism participates in the sensitizing action of estrogens in the pro-apoptotic effect of TNF-α on these cells. Now, we report the effect of estrogens on TNF-α-induced NF-κB nuclear translocation and apoptosis in GH3 cells. As determined by Western blot, estradiol increased nuclear concentration of NF-κB/p105, p65 and p50 in GH3 cells (p