Adult PDAPPJ20 transgenic mice shows increased brain permeability to Evans Blue dye and human brain microvasculature endothelial cells responded to ER induction causing increased permeability in vitro

PRESA J; POMILIO C; ALAIMO A; BENTIVEGNA M; VINUESA A; GREGOSA, AMAL; RAMSHORT R; PEREZ O; BEAUQUIS J; SARAVIA F

Mar del Plata

Congreso; Reunion de la Sociedad Argentina de Investigación Clínica; 2019

SAIC

The blood-brain barrier (BBB) limits flux from and into the brain compartment that is essential for normal neuronal functioning and information processing. Post-mortem tissue analysis indicates BBB damage in Alzheimer?s disease (AD). However, timing and mechanisms underlaying BBB breakdown remain elusive. The endoplasmic reticulum (ER) mediates wide-ranging functions, including protein synthesis, folding, maturation, etc. ER stress is caused by disruption of homeostatic mechanisms that cause unfolded proteins to accumulate. The ER adapts to stress by activating the unfolded protein response (UPR). Chronic ER stress is increasingly being recognized as a common contributor to a wide range of prevalent human diseases, including neurodegeneration.We found augmented vascular permeability in the hippocampus of 12-month-old PDAPPJ20 transgenic mice, model of AD, compared to the non-transgenic group, assessed by Evans Blue i.v. injections and analysis of coronal brain sections (add data p