ROLE OF CERAMIDE SYNTHESIS IN GLIAL ACTIVATION AND COMMUNICATION AFTER A LIPOTOXIC STIMULUS

BELLOTTO, MELINA; BENTIVEGNA, MELISA; GREGOSA, AMAL; POMILIO, CARLOS; GONZALEZ PEREZ N; PRESA, JESSICA; BEAUQUIS JUAN; VINUESA, ANGELES; FLAVIA EUGENIA SARAVIA

Mar del Plata

Congreso; Reunion Anual de la Sociedad Argentina de Investigacion Clinica; 2022

SAIC

Saturated fatty acids (SFA) are basic components of western-style diets. High systemic andcerebral levels promote chronic inflammation and metabolic disorders. This scenario couldlead to brain dysfunction through multiple pathways and be a risk factor for neurodegenerativediseases. Previously, we have shown that a high-fat diet (HFD) induces central and peripheralinflammation, cognitive impairment and hippocampal glial activation, in young and adult mice(Vinuesa et al. 2016, 2019). One of the mediators involved in these pathways are ceramides,lipidic molecules that, besides their physiological roles, could induce inflammation.In the present work, we aimed to study 1) the impact of palmitate (PA), one of the mostabundant SFA in western-style diets, on glial activation and communication and 2) the role ofceramide synthesis mediating the effects of PA.Incubation of microglia (BV2 mouse cell line) with 0.5 uM PA induced NFkB p65 nucleartranslocation (p