Backuping Genomic info from South American endangered Species, Yaguar first on the line.

TARIFA RIESCHLE, INTI; PISCIOTTANO, FRANCISCO; PENNA, CLEMENTINA; SAUZ MARTINEZ, ESTER; GABALDÓN, TONI; SARAGÜETA, PATRICIA

San Pedro de Atacama

Workshop; Exploring Genomic Landscapes: An EMBO Workshop; 2019

EMBO, CGR (Chile)

Backuping Genomic info from South Americanendangered Species, Yaguar first on the line.Inti Tarifa 1 , Fransisco Pisciottano 1 , Clementina Penna 1 , Ester Sauz Martinez 2 ,ToniGabaldon 2 , Patricia Saragüeta 11 Instituto de Biología y Medicina Experimental (IByME)-Buenos Aires, Argentina2 Centre de Regulació Genómica (CRG)-Barcelona, EspañaThe use of next generation sequencing technology has allowed to disassembly genomes ofnew species into parts. Nevertheless knowing the identity of the parts forming thesegenomes still isn't enough to clarify the primary sequence and still less informative in theprocess of reconstructing genomic structure and function.Our project seeks to combine both classical short by Illumina technologie and long readsequencing using Oxford Nanopore Platform (ONT) with approaches that might clarify thespatial genomic conformation.We hope this information leads to a whole new comprehension in genetic circuits. Aiming tosolve questions in a large spectrum of disciplines as differentiation, innate behavior, nongenetic heritage, evolution and illness for example.Being the Jaguar ( Panthera Onca) top specie in its trophic chain its population can beconsidered as an health indicator of its habitat. As its population declines we focused ourefforts on this key predator, aiming to assist conservative works through quality genomicinformation. So far we sequenced nine yaguar individuals through Illumina and one alsothrough ONT. We also sequenced other three novel south american feline species by asecond generation approach allowing evolutionary studies inside this group.The current Panthera Onca genome assembly done with a 84X coverage managed toreconstruct completely 56,05% of genes present. Its using data from long read sequencingthat we expect to generate a second version in which at least 95% of genes may beidentified. Then we aim to construct a third version with chromosome length scaffolds usingHiC technique. Workflow exposed here is then to be applied to other South Americanspecies.This Project is financed by a grant given by SECyT, Research project PICT 2015 3426.2015-2018