Cell-type-specific PR gene regulation in endometrial cancer cells

LA GRECA, ALEJANDRO; BELLORA, NICOLAS; LE DILY, FRANCOIS; QUILEZ OLIETE, JAVIER; JARA, RODRIGO; MERINO, GABRIELA; FRESNO, CRISTOBAL; TARIFA RIESCHLE, INTI; VALLEJO, GRISELDA; VICENT, GUILLERMO; FERNÁNDEZ, ELMER; BEATO, MIGUEL; SARAGÜETA, PATRICIA

Buenos Aires

Congreso; Reunión conjunta de Sociedades de Biociencias; 2017

SAIC

Abstract: Estrogen (E2) and Progesterone (Pg) and their specific receptors ER and PR respectively are major determinants in the development and progression of breast and endometrial malignancies. We have studied the cell specificity of E2 and synthetic progestin R5020 action in human Ishikawa endometrial cancer cell line and compared it to similar genomic strategies in human T47D breast cancer cell line. Both hormones regulate pathways linked to cell proliferation in the breast and endometrial cancer cells, through different gene networks and signaling pathways. Using ChIPseq, we identified ~1,400 binding sites for PR (PRbs) and ~1,900 for ERalfa (ERbs) which are largely specific for Ishikawa and distal (>50kb) to TSS (FDR cutoff for peak calling q = 0.05; FDR cutoff for downstream analysis q