ransient inflammatory gene expression triggers reorganization of chromatin topology associated to decidualization

LUCIANA ANT; JULIETA ERRAMOUSPE; FRANCOIS LE DILY; MIGUEL BEATO; PATRICIA SARAGÜETA

Congreso; EpIC - EpiGene3Sys meets INC-Spain to ChromDesign the Genome; 2022

Endometrial Stromal Fibrobalst (ESFs) differentiation into Decidual Stromal Cells (DSCs) is a crucial step in stablishing and mantaining pregnancy in all eutherian mammals.This process, called decidualization, ocurrs in response to progestin and estrogen levels. Decidualization entails celular and fisiological reprogramming, including dramaticchanges in gene expression, celulllar morphology, vascular remodelling and the transformation of uterin glands. Furthermore, Progesterone not only yelds a stable responsein ESFs, that leads to decidualization, but also a transient response in epithelial cells to regulate estradiol dependent cell proliferation (La Greca et al., 2022).To understand how the changes in gene expression and chromatin landscape unfold during this process, we analyzed the transcriptome and chromatin conformation oftHESC cells, at different time-points after the induction of decidualization. The induction was performed in vitro using two different treatments: cAMP, progesterone (P4)and Estrogen (E2) and cAMP, the synthetic progestin R5020 and E2. We also used EtOH as vehicle control treatment.We did not take into consideration the changes that happened both in treated and control changes, so all the results presented in this poster only take into account thechanges that happen exclusively in decidualized treated cell.