CHROMATIN SPATIAL ORGANIZATION DURING DECIDUALIZATION

ANT L; ERRAMOUSPE J; LE DILY, F; BRUQUE CD; PANIZZO, S; VALLEJO, G; PISCIOTTANO, F; BEATO, M; SARAGÜETA, PATRICIA

Congreso; Reuninón Biociencias 2021-Reunión Conjunta SAIC. SAI. AAFE. NANOMED-AR; 2021

Decidualization is a process of endometrial trans-differentiation crucial to sustain pregnancy. The events involved are regulated by steroid hormones progesterone and estradiol (E2) via the ligand activated transcription factors progesterone receptor (PR) and estrogen receptor (ER). Endometrial specific transcription is also regulated by HOXs and FOXO transcription factors. In this work we used High-throughput Chromosome Conformation Capture (Hi-C) to analyze the changes in chromosome conformation organization and RNA-seq to explore changes in gene expression signatures at different time points of in vitro decidualization of human t-HESC cell line. Decidualization was induced by exposure to cAMP, E2 and either progesterone or the synthetic progestin R5020. PGR and FOXO1, both crucial transcription factors in decidualization, showed >4 fold change increase after 3 days of treatment. On the other hand the decidualization markers, PRL and IGFBP1, both displayed >20 fold change increase after 3 days. At day 1 the inflammatory signature was differentially upregulated (FC>2, FDR