INVESTIGADORES
SANTA MARIA Guillermo Esteban
artículos
Título:
Thylakoid-bound Ascorbate Peroxidase Mutant Exhibits Impaired Electron Transport and Photosynthetic Activity
Autor/es:
C.H DANNA, C. BARTOLI, F. SACCO, L. INGALA, G.E. SANTA-MARIA, J.J. GUIAMET, AND R. A UGALDE
Revista:
PLANT PHYSIOLOGY.
Referencias:
Año: 2003 vol. 132 p. 2116 - 2125
ISSN:
0032-0889
Resumen:
In chloroplasts, stromal and thylakoid-bound ascorbate peroxidases (tAPX) play a major role in the removal of H2O22O2
produced during photosynthesis. Here, we report that hexaploid wheat (Triticum aestivum) expresses three homeologous
tAPX genes (TaAPX-6A, TaAPX-6B, and TaAPX-6D) mapping on group-6 chromosomes. The tAPX activity of a mutant line
lacking TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
lacking TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
tAPX genes (TaAPX-6A, TaAPX-6B, and TaAPX-6D) mapping on group-6 chromosomes. The tAPX activity of a mutant line
lacking TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
lacking TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
Triticum aestivum) expresses three homeologous
tAPX genes (TaAPX-6A, TaAPX-6B, and TaAPX-6D) mapping on group-6 chromosomes. The tAPX activity of a mutant line
lacking TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
lacking TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
TaAPX-6A, TaAPX-6B, and TaAPX-6D) mapping on group-6 chromosomes. The tAPX activity of a mutant line
lacking TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.
TaAPX-6B was 40% lower than that of the wild type. When grown at high-light intensity photosystem II electron
transfer, photosynthetic activity and biomass accumulation were significantly reduced in this mutant, suggesting that tAPX
activity is essential for photosynthesis. Despite the reduced tAPX activity, mutant plants did not exhibit oxidative damage
probably due to the reduced photochemical activity. This might be the result of a compensating mechanism to prevent
oxidative damage having as a consequence a decrease in growth of the tAPX mutant plants.