Bone morphogenetics proteins mediate diabetic intestinal dysfunction

HONORÉ S.M.; GENTA S.B.; VILLECCO E.I.; SÁNCHEZ S.S.

Rosario, Santa Fé, Argentina

Congreso; XLII Annual Meeting Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2006

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Bone morphogenetic proteins (BMPs) have be en shown to be involved in a wide range of cellular behavior from embryogenesis through organogenesis in the gut. In previous work we have demonstrate that this developmental pathway remains in adult life and could be altered in diseased tissue. Gastrointestinal disorders are common complications in diabetic state. In this study we have investigated the implications of BMP/Smad pathway in diabetic intestinal disorders. Our results demonstrate a significative alteration of mRNA profile for BMP ligands, receptors and Smad cytoplasm effectors during diabetic state. We observed change in levels of BMP2, BMP4 and BMP7 mRNA In contrast no variation for BMP6 mRNA was detected. An increased of BMP type 1, 2 receptors and Smad 1 mRNA was al so reported. Interestingly similar results were obtained at protein level by western blotting. Inmunohistochemical labelling reveals altered distribution of BMP ligands BMP2, BMP4 and BMP6 under hyperglycaemia conditions. Also, we have evaluated the level of phosphorilated Smad1 indicative of active BMP/Smad signalling. This protein is down regulated in diabetic animals. In the present work we observed an association between the diabetic intestinal dysfunction with alterations in BMP/Smad signalling, suggesting a role for BMPs in the adult intestinal homeostasis.