Cytotoxic Activity of Thelesperma megapotamicum Organic Fractions against MCF-7 Human Breast Cancer Cell Line

FIGUEROA, ANA; SORIA, ELIO; CANTERO, JUAN; SANCHEZ, MÓNICA; GOLENIOWSKI, MARTA

Journal of Cancer therapy

Scientific Research Publishing

Año: 2011

2151-1934

Thelesperma megapotamicum (Asteraceae) is commonly used in Argentine to treat various diseases (renal, digestive affections, and as anaesthesia). The present study showed the mechanisms involved "in vitro" cytotoxicity of T. mega- potamicum Fractions. Five Fractions (F1 - F5) were separated by column chromatography (Silica gel) using hex-ane:diethyl ether as eluents. Viability was evaluated in Human breast carcinoma cell line (MCF-7) by staining with crystal violet. With respect to F1 Fraction treatment, the cell survival was 49.14% ± 8.87%, while the F2 and F3 ones exhibited a strong reduction of cell viability to only 26.35% ± 1.63% and 23.3%1 ± 0.53% of the control cell at 50 ìg/ml, respectively. Apoptotic effect of these Fractions was detected using FITC-labeled Annexin V and propidium iodide binding assays and was confirmed by a higher proportion of apoptotic cells due to F2 and F3 treatments. T. megapotamicum active Fractions could facilitate the tumoral cells death by decreasing the activity of the enzyme Gamma-glutamyltranspeptidase and causing alteration in cell membrane sialoglycoconjugates and others involved anti-cancer mechanisms including apoptosis.