In Vitro Screening of Plant Extract: Neurotoxic Effects of the “Sierras de

LANDA, CARLOS; SANCHEZ, MÓNICA

Molecular Medicinal Chemistry

INSTITUTO DE INVESTIGACIÓN DE LAS CIENCIAS EXACTAS FÍSICAS Y NATURALES

Año: 2010 vol. 20 p. 48 - 52

1666-888X

To investigate the n e u r o toxic effect of some “Si e r r a s de Córdoba ” plant extracts, three plants from this place were extracted with ethylic ether, ethanol and milliQ-water. The effects of different extracts on early events in the normal development of neuron were studied using cultured embryonic rat hippocampal cells, which develop in vitro in a stereotypical sequence of events that mimics their development in vivo. In addition, the neuron viability was evaluated using lactate dehydrogenase (LDH) assay. Two ethylic ether, two aqueous extracts from Thelesperma Megapotamicum and Armeniaca Vulgaris and one ethanolic on early events in the normal development of neuron were studied using cultured embryonic rat hippocampal cells, which develop in vitro in a stereotypical sequence of events that mimics their development in vivo. In addition, the neuron viability was evaluated using lactate dehydrogenase (LDH) assay. Two ethylic ether, two aqueous extracts from Thelesperma Megapotamicum and Armeniaca Vulgaris and one ethanolic ethylic ether, ethanol and milliQ-water. The effects of different extracts on early events in the normal development of neuron were studied using cultured embryonic rat hippocampal cells, which develop in vitro in a stereotypical sequence of events that mimics their development in vivo. In addition, the neuron viability was evaluated using lactate dehydrogenase (LDH) assay. Two ethylic ether, two aqueous extracts from Thelesperma Megapotamicum and Armeniaca Vulgaris and one ethanolicextracts from Thelesperma Megapotamicum and Armeniaca Vulgaris and one ethanolic extract (Armeniaca Vulgaris) showed no toxicity a g a i n s t embryonic rat hippocampal cells in culture. The ethanolic extract of Thelesperma Megapotamicum showed a slight (but no significative) toxicity against the same neuronal cells. While, the three extract studied of Aristolochia Stuckertii Speg, at both doses, showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the same neuronal cells. While, the three extract studied of Aristolochia Stuckertii Speg, at both doses, showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect The ethanolic extract of Thelesperma Megapotamicum showed a slight (but no significative) toxicity against the same neuronal cells. While, the three extract studied of Aristolochia Stuckertii Speg, at both doses, showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the same neuronal cells. While, the three extract studied of Aristolochia Stuckertii Speg, at both doses, showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect embryonic rat hippocampal cells in culture. The ethanolic extract of Thelesperma Megapotamicum showed a slight (but no significative) toxicity against the same neuronal cells. While, the three extract studied of Aristolochia Stuckertii Speg, at both doses, showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the same neuronal cells. While, the three extract studied of Aristolochia Stuckertii Speg, at both doses, showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect he ethanolic extract of Thelesperma Megapotamicum showed a slight (but no significative) toxicity against the same neuronal cells. While, the three extract studied of Aristolochia Stuckertii Speg, at both doses, showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect Aristolochia Stuckertii Speg, at both doses, showed high toxicity against the rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect rat hippocampal cells in culture. Taken together, our results describes, for the first time, that Aristolochia Stuckertii Speg have neurotoxic effects. Further intensive studies are required to clarify how Aristolochia Stuckertii Speg produces this effect