INVESTIGADORES
RUIZ Oscar Adolfo
congresos y reuniones científicas
Título:
EVALUATION OF RELATIONSHIP BETWEEN PUTRESCINE AND ABA IN Arabidopsis thaliana
Autor/es:
ALET A.I.; SANCHEZ D.H.; CUEVAS J.C.; CARRASCO, P.; ALTABELLA, RUIZ O.A
Lugar:
Rosario. Argentina
Reunión:
Congreso; Congreso XLII Reunión Anual SAIB; 2006
Institución organizadora:
SAIB
Resumen:
Putrescine (Put), spermidine (Spd), and spermine (Spm) form a
class of aliphatics amines that are ubiquitous in living organisms,
and have been implicated in a wide range of biological
processes, including plant growth and development. However,
their mode of action remains a matter of speculation. In recent
years, attention has been focused on the involvement of PAs in
response to environmental stresses. In plants PAs biosynthesis
begins principally with Arg decarboxylation by Arginine
decarboxylase (ADC; EC 4.1.1.19). There are general agreement
in consider this enzyme as a key enzyme playing a decisive role
in the regulation of the PAs levels. On the other hand, abscisic
acid (ABA) appears to play an important role in plant response to
these stresses. In orther to further analyze PAs involvement in
abiotic stresses and their relationship with stress hormone ABA,
we obtained transgenic Arabidopsis thaliana plants expressing
oat ADC under the stress inducible promoter rd29A. When
treated with ABA or acclimated to cold, differences in the
expression of genes involved in stress response (analyzed by
real-time PCR) were observed. We also evaluated the
performance of these plants under freezing and dehydration
stresses, suggesting that endogenous Put levels could be
involved in the tolerance mechanism and the different gene
expression patterns observed in the transgenic plants.