2. Oxidation of N-alkyl and C-alkylputrescines by diamine oxidases.

ROSALÍA B. FRYDMAN. OSCAR A. RUIZ. MIRIAM KREISEL AND URIEL BACHRACH.

FEBS LETTERS

Año: 1987 vol. 219 p. 380 - 386

0014-5793

N-Methyl-, N-ethyl-, N-propyl- and N-butylputrescine were assayed as substrates of diamine oxidases from pea seedling and pig kidney. With the exception of N-methylputrescine they were found to be oxidized to the corresponding aminoaldehydes. l-Methyl-, 2-methyl-, l-ethyl- and 1-propylputrescine were oxidized by the oxidases at lower rates than the N-alkylderivatives. 1,3_Dimethylputrescine had negligible oxidation rates while 1,4_dimethylputrescine (2,5-diaminohexane) was not a substrate. The oxidation of putrescine by the kidney oxidase was inhibited by 1,4_dimethylputrescine, while the pea oxidase was strongly inhibited by the former as well as by 2-methylputrescine and 1,3_dimethylputrescine. Serum amine oxidase did not oxidize the substituted putrescines although several of the latter inhibited spermidine oxidation by this oxidase.