Synthesis of very long chain (up to 36 carbon) tetra, penta and hexaenoic fatty acids in retina

ROTSTEIN, N.P.; AVELDAÑO, M.I.

BIOCHEMICAL JOURNAL

PORTLAND PRESS LTD

Año: 1988 vol. 249 p. 191 - 200

0264-6021

The synthesis of very long chain (C24 to C36) polyunsaturated (four, five and six double bonds) fatty acids (VLCPUFA) is investigated in bovine retina using [14C]acetate. Saturates on the one hand (mainly palmitate), and polyenes on the other (mainly VLCPUFA), incorporate most of the label found in lipids. Phosphatidylcholine (PC) is the most highly labelled lipid class, since both types of ´4C-labelled fatty acids, but especially this novel series of VLCPUFA, are concentrated in this phospholipid. Radioactivity from [14C]acetate is found in very long chain tetra, penta and hexaenoic fatty acids of PC. The labelling of 20: 4(n -6), 20: 5(n -3), 22: 5(n -6) and 22: 6(n -3) is much lower than that of longer polyenes of each of these series, indicating that VLCPUFA are synthesized in situ by successive elongations of the above polyenes, pre-existing in retina lipids. In various subcellular fractions isolated from retinas after incubations with [14C]acetate (including cytosol, microsomes, mitochondria and photoreceptor membranes), the labelling of the VLCPUFA of PC is very high, even at relatively short intervals of incubation. The resultssuggest that not only the synthesis but also the intracellular traffic among membranes of VLCPUFAcontaining species of PC are very active processes in the retina.