INVESTIGADORES
ROMANO Maria Isabel
artículos
Título:
Molecular typing of Mycobacterium bovis isolates in Argentina: first description of a person-to-person transmission case
Autor/es:
ETCHECHOURY, G ECHEVERRÍA VALENCIA, N MORCILLO, M SEQUEIRA, B IMPERIALE, M LÓPEZ, K CAIMI, M ZUMÁRRAGA, A CATALDI, AND M ROMANO.
Revista:
Zoonoses Public Health
Editorial:
ISSN1863-1959 Edited by:Mary Torrence
Referencias:
Año: 2010 vol. 57 p. 375 - 381
Resumen:
Bovine tuberculosis is caused by Mycobacterium bovis, a mycobacterium highly
similar to M. tuberculosis that belongs to the M. tuberculosis complex. The main
host of M. bovis is cattle but it also affects many other mammalians including
humans. Tuberculosis in humans caused by either M. bovis or M. tuberculosis
is clinically hard to distinguish. During 20042005, samples from 448 patients
with diagnosis of TB were collected from different regions of Argentina. The
PRA technique identified 400 isolates with representative patterns of mycobacterium.
The predominant ones were the M. tuberculosis complex, the M. avium
M. intracellulare complex and M. gordonae. Samples with M. tuberculosis
complex PRA restriction profiles were analyzed with a multiplex PCR to differentiate
between M. tuberculosis and M. bovis. Multiplex PCR identified nine
M. bovis. The results allowed the possibility to establish that 2% of pulmonary
tuberculosis was due to M. bovis. Isolates of M. bovis from humans were examined
using spoligotyping. These isolates presented five different spoligotypes.
The main spoligotype was also the most frequently one found in cattle. The
remaining human spoligotypes (grouped in clusters) are occasionally found in
cattle. Variable number tandem repeat (VNTR) analysis identified five different
patterns. By combining the results of spoligotyping and VNTR analysis, we
were able to differentiate seven M. bovis isolates. The remaining two M. bovis
samples showed the same spoligotype and VNTR profile and belonged to
household contacts. An MDR-M. bovis was isolated from the samples of these
household contacts. The identification of two epidemiologically linked cases
of human M. bovis infection suggests person-to-person transmission of an
MDR-M. bovis.