Genetic diversity of Mycobacterium avium sp. paratuberculosis by mycobacterial interspersed repetitive Unit-Variable number tandem repeat and multi-locus short-sequence repeat one-sentence summary: Genetic diversity of Mycobacterium avium sp. paratubercul

ROBERTO DAMIÁN MOYANO, BELÉN ROCÍO IMPERIALE, MAGALÍ ROMERO, MARÍA DE LA PAZ SANTANGELO, MARÍA FIORELLA ALVARADO PINEDO, GABRIEL TRAVERÍA, MARÍA ISABEL ROMANO

International Journal of Mycobacteriology

Asian African Society of Mycobacteriology,

Año: 2021 vol. 10 p. 51 - 59

2212-5531

Background:ParatuberculosisisanentericdiseasecausedbyMycobacteriumaviumsp.paratuberculosis(MAP)thataffectsmainlyruminantproducinglossestothelivestockindustry.ManymolecularepidemiologicalmethodshavebeenusedtodiscriminateMAPisolates.Method:TheaimofthisstudywastodescribethegeneticdiversityoftheArgentineanMAPisolatesusingacombinationoftwomolecularsystems,themycobacterialinterspersedrepetitiveunit?variablenumbertandemrepeat(MIRU‑VNTR)(?automatedand?non‑automated?)andthemulti‑locusshort‑sequencerepeat(MLSSR)system.Results:Thirty‑twoisolateswereidentifiedasMAPofCtypebyIS900polymerasechainreaction(PCA)andIS1311PCA‑restrictionenzymeanalysis.ThemainpatternsfoundbybothMIRU‑VNTRsystemswereINMV1(54.5%),INMV2(24.2%)andINMV11(9.1%).TheINMV5,INMV8andINMV16wererepresentedwithoneisolateeach(3.0%).Only4MIRU‑VNTRlociwerepolymorphic.Conclusion:ThoseisolatessharingthesameINMVpatternswereanalyzedbyMLSSR,beinglocus2themostpolymorphiconeshowingisolateswith9,10,11,andmorethan11?G?repeats.Besides,theglobaldiscriminatorypoweramongisolatescouldbeincreasedusingbothtechniques.Basedontheseresults,ashortversionofthe?automated?MIRU‑VNTRcouldbeusedasascreeningtooltogroupisolatesgeneticallyrelatedandsubsequentlyperformtheSSRusinglocus2onthoseisolatessharingthesameINMVpattern.