INVESTIGADORES
RODRIGUEZ TALOU Julian
congresos y reuniones científicas
Título:
Dengue protein production in plant using Agrobacterium-mediated and biolistic transformation system
Autor/es:
MARTÍNEZ CA; PERASSOLO M; CARDILLO AB; BUSTO, V.; QUEVEDO CV; GIULIETTI AM; RODRÍGUEZ TALOU J
Lugar:
Carlos Paz, Cordoba
Reunión:
Congreso; XLIV Congreso de SAIB,; 2008
Institución organizadora:
SAIB
Resumen:
Production of recombinant proteins in plant systems has emerged as a new alternative platform. Plant cells can be maintained in simple and economic media, without risk of contamination with bacterialtoxins, virus or prions. Moreover, the plant cells can glycosilate and do the post-transcriptional arrangements need for complex glycoproteins. The envelope protein (E) is the major structural component and the most immunogenic ofdengue virus proteins and it is involved in the induction of a protective immunity. E protein needs to be directed to the secretory pathway through the N-terminus signal peptide (SP), to be N-glycosilated.The aim of this work is to produce E protein in plant systems to serve as a diagnostic reagent in the rapid detection of dengue virus. A gene encoding dengue type 2 E protein was successfully cloned in a binary vector and expressed in Nicotiana tabacum and Morinda citrifolia cell suspension cultures using Agrobacterium-mediated expression systems. The result indicates that expression system used can produce the dengue virus antigenin plant. We are currently evaluating the integrity and the expressions level of this protein. The effect of different genetic sequences on the expression levels of the E protein will be assessed.Nicotiana tabacum and Morinda citrifolia cell suspension cultures using Agrobacterium-mediated expression systems. The result indicates that expression system used can produce the dengue virus antigenin plant. We are currently evaluating the integrity and the expressions level of this protein. The effect of different genetic sequences on the expression levels of the E protein will be assessed.
rds']