RODRIGUEZ TALOU Julian
capítulos de libros
Isochorismate synthase overexpression in Catharanthus roseus cell suspensions
RODRIGUEZ TALOU , J; VERBERNE, M C; BUDI MULJONO, RA; VAN TEGELEN, LJP; GONSALVEZ BERNAL, B; LINTHORST, HJM; WULLEMS, GJ; VERPOORTE, R
Introducing the Microbial Salycilic Acid Pathway into Plants. Influence on Plant Expression and Pathogen Resistance
Lugar: Leiden; Año: 2000; p. 33 - 44
Abstract Cell suspensions of Cathavanthus roseus produce 2,3-dihydroxybenzoic acid (2,?-DHBA) after elicitation with fungal elicitor. Together with the 2,3-DHBA production there is a strong increase in isochorismate synthase (ICS) activity, an enzyme whch converts ckorismate in to isochorismate, Bacteria also produce 2,3-DHBA which is an intermediate for the biosynthesis of siderophores. The biosynthesis pathway of 2,3 -DHBA in bacteria proceeds via ICS. To investigate whether C. roseus cells use the same pathway to produce 2,3-DHB A, cells were transformed with constructs containing the ics cDNA clone in sense or antisense orientation. Although the transformation with ics in sense orientation resulted in constitutive ICS activity, no accumulation of 2,3-DHBA was observed. Transformation of ics in antisense orientation was not sufficient to block the ICS production. The biosynthesis of 2,3-DHBA in C. raseus probably involves a multi-enzyme pathway, which cannot simply be modulated by overexpression of ics.