Turnover rates for glycerol, acetate and orthophosphate in phospholipids of the rat cerebral cortex

E. LAPETINA; G. RODRÍGUEZ DE LORES ARNAIZ; E. DE ROBERTIS

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 1969 vol. 176 p. 643 - 646

0005-2736

Previous studies have demonstrated that phospholipids are the major lipid constituents of the various membranous fractions of brain, i.e., myelin, synaptic vesicles, nerve-ending membranes, mitochondria and microsomes. It was also found that when [32P]orthophosphate was administered intracranially, the phospholipids of all these fractions became labelled at the same ratea. These findings and some reports from the literature indicating that in brain and liver the turnover times of different parts of the phospholipid molecule could vary led us to investigate this problem using several labelled precursors. The results of this investigation showed that the half-lives of phospholipids of the cerebral cortex vary considerably when studied with the precursors [32P]orthophosphate, [14C]acetate and [14C]glycerol. This suggests that the various portions of the phospholipid molecule may be interchanged or metabolized at different rates. Adult Wistar rats of about 100g were injected intraventricularly.