CONICET | Buscador de Institutos y Recursos Humanos

SINCEB the first description of synaptic vesicles as a specific component of synaptic endings it vas suggested that they could be associated with acetylcholine (ACh) and other chemical transmitters . The recent development of a technique for the separation of such vesicles from isolated nerve endings paved the way to demonstrate that they indeed contain the transmitter in multimolecular or quantal units . The method used to "microdissect" the synaptic complex consists in theswelling and bursting by osmotic shock of the membrane limiting the ending with liberation of the synaptic vesicles and mitochondria contained within. A fraction containing, among other components, the membranous synaptic ghosts and another rich in synaptic vesicles were then separated by differential centrifugation .Although this treatment caused considerable liberation of the bound ACh, this transmitter, as well as the synthesizing enzyme choline acetylase were found highly concentrated in the vesicular fraction of the rat brain

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