Clozapine administration modifies neurotensin effect on synaptosomal membrane Na+, K+ -ATPase activity.

M. G. LÓPEZ ORDIERES; G. RODRÍGUEZ DE LORES ARNAIZ

NEUROCHEMICAL RESEARCH

SPRINGER/PLENUM PUBLISHERS

Año: 2009 vol. 34 p. 2226 - 2232

0364-3190

Na+, K +-ATPase is inhibited by neurotensin, an effect which involves the peptide high affinity receptor (NTS1). Neurotensin effect on cerebral cortex synaptosomal membrane Na+, K +-ATPase activity of rats injected i.p. with antipsychotic clozapine was studied. Whereas 3.5 x 10-6M neurotensin decreased 44% Na+, K +-ATPase activity in the controls, the peptide failed to modify enzyme activity thirty min after a single 3.0, 10.0 and 30.0 mg/kg clozapine dose. Neurotensin decreased Na+, K+-ATPase activity 40% or 20% eighteen hours after 3.0 or 5.6 mg/kg clozapine administration, respectively, and lacked inhibitory effect eighteen hours after 17.8 and 30.0 mg/kg clozapine doses. Results indicated that the clozapine treatment differentially modifies the further effect of neurotensin on synaptosomal membrane Na+, K+-ATPase activity according to time and dose conditions employed. Taken into account that clozapine blocks the dopaminergic D2 receptor, findings obtained favor the view of an interplay among neurotensinergic receptor, dopaminergic D2 receptor and Na+, K+-ATPase at synaptic membranes.