Basal FSH and peak gonadotropin levels following GnRH infusion show high diagnostic accuracy in boys with suspicion of hypogonadotropic hypogonadism

GRINSPON, R.; ROPELATO, M.G.; GOTTLIEB, S; KESELMAN, A.; MART¨ªNEZ, A.; BALLERINI, M.G.; DOMEN¨¦, H.; REY, R

JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM

ENDOCRINE SOC

Año: 2010 vol. 95 p. 2811 - 2818

0021-972X

Context: Differential diagnosis between hypogonadotropic hypogonadism (HH) and constitutional delay of puberty in boys is challenging. Most tests use an acute GnRH stimulus, allowing only the release of previously synthesized gonadotropins. A constant GnRH infusion, inducing de novo gonadotropin synthesis, may allow a better discrimination. Objective: We evaluated the diagnostic accuracy of basal and peak gonadotropins after GnRH infusion, measured by ultrasensitive assays, to confirm the diagnosis in boys with suspected HH. Design and Setting: We conducted a validation study following Standards for Reporting of Diagnostic Accuracy criteria at a tertiary public hospital. Patients and Methods: A GnRH iv infusion test was performed in 32 boys. LH and FSH were determined by immunofluorometric assay at 0?120 min. Diagnosis Ascertainment: The following diagnoses were ascertained: complete HH (n_19; testes_4 ml at 18 yr), partial HH (n _ 6; testes enlargement remained arrested for _1 yr or did not reach 15 ml), and constitutional delay of puberty (n _ 7; testes _ 15 ml at 18 yr). Main Outcome Measures: Sensitivity, specificity, positive and negative predictive values, and diagnostic efficiency were assessed. Results: Basal FSH less than 1.2 IU/liter confirmed HH with specificity of 1.00 (95% confidence interval _ 0.59?1.00), rendering GnRH infusion unnecessary. In patients with basal FSH of at least 1.2 IU/liter, the coexistence of peak FSH less than 4.6 IU/liter and peak LH less than 5.8 IU/liter after GnRH infusion had high specificity (1.00; 95% confidence interval _ 0.59?1.00) and diagnostic efficiency (76.9%) for HH. Conclusions: Basal FSH less than 1.2 IU/liter confirms HH, which precludes from further testing, reducing patient discomfort and healthcare system costs. In patients with basal FSH of at least 1.2 IU/liter, a GnRH infusion test has a high diagnostic efficiency.