Extracellular phospholipids of sunflower seeds are modulated by ABA and jasmonic acid

CORTI-MONZÓN, G.; MARIANA CLELIA REGENTE; DE-LA-CANAL, L.

Mar del Plata

Congreso; XLIII Reunión Anual de la SAIB; 2007

Extracellular phospholipids of sunflower seeds are modulated by ABA and jasmonic acid. Corti Monzón G, Regente M, de la Canal L. Instituto de Investigaciones Biológicas -FCEyN- Universidad Nacional de Mar del Plata. Mar del Plata Phospholipids (PLs) are well known intracellular messengers involved in plant development and stress responses. We have recently reported the presence of PLs in extracellular washing fluids (EWF) of sunflowers seeds, demonstrating that phosphatidic acid (PA) and phosphatidylinositol (PI) are the major components of this fraction. This finding has suggested their putative involvement in intercellular signaling events. The aim of this work was to determine if plant hormones involved in seed germination and seedling growth were able to induce changes in the composition of extracellular phospholipids. PLs levels in EWF were determined by electrospray ionization tandem mass spectrometry and lipid overlay assays. Imbibition of sunflower seeds with 100 ¦ÌM jasmonic acid (JA) induces a decrease in PI content and accumulation of phosphatidylinositol 4-phosphate (PI4P) and specific PA species in EWF. On the other hand, the EWF from seeds treated with to 50 ¦ÌM abscisic acid (ABA) exhibit an increase in PA and phosphatidylglycerol levels. The existence of signaling PLs such as PA, PI and PI4P as extracellular components of seeds and the modulation of their levels by hormonal treatments suggest their contribution to intercellular communication in planta.