INTERACTION OF HELJA LECTIN ISOLATED FROM HELIANTHUS ANNUUS WITH CANDIDA ALBICANS INHIBITS PHAGOCYTOSIS AND PROMOTES PHENOTYPIC MATURATION IN DENDRITIC CELLS.

CHOP, MAIA; RADICIONI, MELISA BELÉN; DEL RIO M; RODRÍGUEZ RODRIGUES, CHRISTIAN; REGENTE, MARIANA

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIAS 2022; 2022

Background: Helja is a sunflower lectin that recognizes mannose motifs. Candidiasis is a fungal infection caused by Candida spp. that affects immunocompromised individuals. This opportunistic yeast can be recognized by dendritic cells (DCs). The aim of this work is to analyze if the interaction between Helja and Candida albicans (Ca) cell wall could inhibit recognition and phagocytosis by DCs and modulate cell maturation. Methods: Bone marrow dendritic cells (BMDCs) were cultured in RPMI 1640 medium supplemented with FLT3-L. Helja was purified from sunflower seeds with a D-mannose-agarose resin. Phagocytosis assay was evaluated by FACS and Confocal Microscopy. C. albicans (NGY152 strain) were stained with FITC and then incubated with Helja for 45 minutes prior to BMDC stimulation. LPS (100 ng/ml) and 4°C incubation were used as controls. BMDCs phenotype was analyzed by FACS. Results: Confocal images and quantification of labeled-yeast revealed that the preincubation of fungal cells with Helja inhibited recognition by BMDCs and induced a statistically significant reduction on phagocytosis compared to control (n=2, ****p