Oligobodies: bench made synthetic antibodies.

RADRIZZANI M; BROCCARDO M; GONZALEZ SOLVEYRA C; REYES GB; CAFFERATA EG; SANTA-COLOMA TA

MEDICINA (BUENOS AIRES)

Fundación Revista Medicina (Buenos Aires)

Lugar: Buenos Aires; Año: 1999 vol. 58 p. 753 - 758

0025-7680

Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as "synthetic antibodies". The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named "oligobodies", has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies: their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA sequence from a database provides enough information to make them.