A single-step, rapid, and versatile method for simultaneous detection of cell surface glycan profiles using fluorochrome-conjugated lectins

NICOLAS TORRES; MONTANA MANSELLE COCCO; RAMIRO PERROTTA; YAMIL MAHMOUD; MARIANA SALATINO; KARINA MARIÑO; GABRIEL RABINOVICH

GLYCOBIOLOGY

OXFORD UNIV PRESS INC

Lugar: Oxford; Año: 2023

0959-6658

Cell surface glycans play essential roles in diverse physiological and pathological processes and their assessment has importantimplications in biomedicine and biotechnology. Here we present a rapid, versatile, and single-step multicolor flow cytometry methodfor evaluation of cell surface glycan signatures using a panel of selected fluorochrome-conjugated lectins. This procedure allowssimultaneous detection of cell surface glycans with a 10-fold reduction in the number of cells required compared with traditionalmultistep lectin staining methods. Interestingly, we used this 1-step lectin array coupled with dimension reduction algorithms in aAQ13 proof-of-concept application for discrimination among different tumor and immune cell populations.Moreover, this procedurewas alsoable to unveil T-, B-, and myeloid cell subclusters exhibiting differential glycophenotypes. Thus, we report a rapid and versatile lectincytometry method to simultaneously detect a particular repertoire of surface glycans on living cells that can be easily implemented indifferent laboratories and core facilities.