Mouse Immunoglobulin Subclasses. Cyanogen Bromide Fragments and Partial Sequence of a gamma 1 Chain

ADETUGBO K; POSKUS E; SVASTI J; MISTEIN C

BIOCHEMICAL JOURNAL

PORTLAND PRESS LTD

Año: 1975 vol. 56 p. 503 - 519

0264-6021

The purification and characterisation of all the cyanogen bromide fragments of MOPC 21 heavy(gamma l) chain is described. The ten BrCN fragments account for the whole chain. Four of thesefragments have been used to establish the sequence of the C-terminal stretch (138 residues) that includes the entire CH3 and a part of the CH2 homology regions. A comparison of this sequence with homologous sequences is presented. Mouse gamma l and gamma 2 proteins differ much more than the gamma subclasses in humans and in other species. The comparison further suggests that the four human gamma subclasses and mouse gamma l have a common ancestor which differs from the mouse gamma 2 ancestor. Unlike other subclasses, mouse gamma l and gamma 2 genes have diverged before speciation. l and gamma 2 proteins differ much more than the gamma subclasses in humans and in other species. The comparison further suggests that the four human gamma subclasses and mouse gamma l have a common ancestor which differs from the mouse gamma 2 ancestor. Unlike other subclasses, mouse gamma l and gamma 2 genes have diverged before speciation. Nota: Este trabajo se encuentra entre las 24 publicaciones en revistas internacionales con referato entre 1969 y 1987 y otras 12 publicaciones de divulgación nacional entre 1983 y 1990, registradas en el CV (Adjunto a SIGEVA CONICET).