Role of the SR-microXRF methodology in the assessment of protective capacity of antioxidant therapy during As exposure

RUBATO BIRRI, P.N.; PEREZ, R.D; PéREZ C.A.; RUBIO M.

Activity Report/Brazilian Synchrotron Light Laboratory

Laboratório Nacional de Luz Síncrotron (LNLS)

Lugar: Campinas; Año: 2009 vol. 2008 p. 1 - 2

1518-0204

Arsenic is a carcinogen present in many aquifers of Argentina and other countries and chronic As consumption induces As and Cu renal cortex accumulation, which was corroborated By SR-ìXRF (line D09B of LNLS). In this regard, As was accumulated in renal glomerulus and peri-glomerular zone of rats who drank 50 ppm As over 60 days and an increase in the Cu concentration that co-located with As. Arsenite (AsIII) binds to macromolecular thiols, causing oxidative stress and subsequent cellular responses implicated in its toxicity and carcinogenicity. Moreover, oxidative damage participates critically in several chronic renal pathologies, involving reactive oxygen species (ROS) production, redoxrelated gene expression and cytotoxity. Since the use of antioxidant plant extracts has been proposed as beneficial. Argentinian medicinal plants was assayed on arsenite-induced oxidative stress in Vero cells (monkey kidney cells). Then, the rats were co-treated with As and antioxidant extract during 60 days and a decrease of up to 30% of As and Cu retention was observed by SR-ìXRF (LNLS, line D09B). Consequently, we speculate that the use of this antioxidant could reduce the toxic effects of As. OBJECTIVES The aim of the present study was to elucidate the protective effect of a plant antioxidant against the arsenite induced As and Cu accumulation in renal cortex.ìXRF (line D09B of LNLS). In this regard, As was accumulated in renal glomerulus and peri-glomerular zone of rats who drank 50 ppm As over 60 days and an increase in the Cu concentration that co-located with As. Arsenite (AsIII) binds to macromolecular thiols, causing oxidative stress and subsequent cellular responses implicated in its toxicity and carcinogenicity. Moreover, oxidative damage participates critically in several chronic renal pathologies, involving reactive oxygen species (ROS) production, redoxrelated gene expression and cytotoxity. Since the use of antioxidant plant extracts has been proposed as beneficial. Argentinian medicinal plants was assayed on arsenite-induced oxidative stress in Vero cells (monkey kidney cells). Then, the rats were co-treated with As and antioxidant extract during 60 days and a decrease of up to 30% of As and Cu retention was observed by SR-ìXRF (LNLS, line D09B). Consequently, we speculate that the use of this antioxidant could reduce the toxic effects of As. OBJECTIVES The aim of the present study was to elucidate the protective effect of a plant antioxidant against the arsenite induced As and Cu accumulation in renal cortex.