Multiememental analysis and arsenic determination in renal cortex of rats by m-SRXRF

PÉREZ R. D.; RUBATTO BIRRI P. N.; PÉREZ C. A.; RUBIO M.; BONGIOVANNI G. A.

Activity Report 2006/Brazilian Synchrotron Light Laboratory

Brazilian Association for Synchrotron Light Technology

Lugar: Campinas (Brasil); Año: 2007 p. 1 - 2

1518-0204

Arsenic is an environmental toxicant and a human carcinogen. The kidney´s proximal tubules are known to be especially sensitive to injury, primarily because of their characteristically high reabsorptive activity. However, a histological examination of As distribution has not been made. In order to determinate As accumulation in urinary system-related organs, a multielemental mapping was performed by m-SRXRF at the Synchrotron Light National Laboratory (Brasil). For that, the kidneys and bladder from rats drinking arsenical water (50 ppm) during 0 or 60 days were removed, snap frozen in liquid nitrogen and then cut in slices of 200 micrometer thickness. The slices were adhered to Ultralene film and were scanned with a collimated white synchrotron spectrum (30 micrometer X 30 micrometer). We found the highest arsenic concentration in peri-glomerular region from kidney, but not As was found in bladder tissues. Additionally, the proportion of other elements also was altered by the chronic exposures to As. The results revealed a tissue-specific arsenic accumulation and suggest a proximal tubule altered reabsorption.