Stability evaluation of polymer-liposome complexes for drug delivery systems prepared with bacterial lipids.

ALVES, P; HUGO, A. A; TYMCZYSZYN, E. E; SZYMANOWSKI, F; PÉREZ, P. F; COELHO, J. F. J; SIMOES, P. N; GÓMEZ-ZAVAGLIA, A.

Conferencia; 12th International Chemical and Biological Engineering Conference. CHEMPOR; 2014

The physicochemical characterization of polymer-liposome complexes (PLCs) prepared with lipids of lactic acid bacteria (Lactobacillus delbrueckii subsp. lactis CIDCA 133) and cholesterol-poly(N,N-dimethylaminoethyl methacrylate) (CHO-PDMAEMA) was studied. PLCs were prepared with different polymer:lipid molar ratios. The optimal polymer:lipid ratio and the stability of both bare liposomes and PLCs were evaluated at 37 °C and at different pHs, as well as after preservation at 4 °C, ?80 °C and freeze-drying in the presence or absence of 250 mM trehalose. In vitro studies on Raw 264.7 and Caco-2/TC7 cells demonstrated an efficient incorporation of PLCs into the cells, preparations with higher stability being better incorporated.