INVESTIGADORES
PEREZ Pablo Fernando
artículos
Título:
Co-culture with potentially probiotic microorganisms antagonises virulence factors of Clostridium difficile in vitro.
Autor/es:
TREJO, F. M.; PÉREZ, P. F. AND DE ANTONI, G. L.
Revista:
ANTON LEEUW INT. J. G.
Editorial:
SPRINGER
Referencias:
Año: 2010 vol. 98 p. 19 - 29
ISSN:
0003-6072
Resumen:
Abstract Toxigenic strains of Clostridium difficileToxigenic strains of Clostridium difficile
were co-cultured with different strains of bifidobacteria
and lactobacilli. Spent culture supernatants were
tested for biological activity on cultured Vero cells.
Co-culture of C. difficile with some potentially
probiotic strains lead to a reduction of the biological
activity of spent culture supernatants. The observed
effects cannot be ascribed either to secreted factors
from the probiotic strains or to toxin adsorption by
bacterial cells. Immunological assays showed that
there was significant diminution of both clostridial
toxins (TcdA and TcdB) in spent culture supernatants
of co-cultures as compared with pure clostridial
cultures. Even though co-cultured clostridial cells
showed a slight increase of intracellular toxins, this
increase did not completely explains the reduction of
toxin concentration in culture supernatants. The
evidence suggests that the antagonism could be due
to the diminution of the synthesis and/or secretion of
both clostridial toxins. Our findings provide new
insights into the possible mechanisms involved in the
protective effect of probiotics in the context of C.
difficile infection.
difficile infection.
difficile infection.
probiotic strains lead to a reduction of the biological
activity of spent culture supernatants. The observed
effects cannot be ascribed either to secreted factors
from the probiotic strains or to toxin adsorption by
bacterial cells. Immunological assays showed that
there was significant diminution of both clostridial
toxins (TcdA and TcdB) in spent culture supernatants
of co-cultures as compared with pure clostridial
cultures. Even though co-cultured clostridial cells
showed a slight increase of intracellular toxins, this
increase did not completely explains the reduction of
toxin concentration in culture supernatants. The
evidence suggests that the antagonism could be due
to the diminution of the synthesis and/or secretion of
both clostridial toxins. Our findings provide new
insights into the possible mechanisms involved in the
protective effect of probiotics in the context of C.
difficile infection.
difficile infection.
difficile infection.
probiotic strains lead to a reduction of the biological
activity of spent culture supernatants. The observed
effects cannot be ascribed either to secreted factors
from the probiotic strains or to toxin adsorption by
bacterial cells. Immunological assays showed that
there was significant diminution of both clostridial
toxins (TcdA and TcdB) in spent culture supernatants
of co-cultures as compared with pure clostridial
cultures. Even though co-cultured clostridial cells
showed a slight increase of intracellular toxins, this
increase did not completely explains the reduction of
toxin concentration in culture supernatants. The
evidence suggests that the antagonism could be due
to the diminution of the synthesis and/or secretion of
both clostridial toxins. Our findings provide new
insights into the possible mechanisms involved in the
protective effect of probiotics in the context of C.
difficile infection.
difficile infection.
difficile infection.
C. difficile with some potentially
probiotic strains lead to a reduction of the biological
activity of spent culture supernatants. The observed
effects cannot be ascribed either to secreted factors
from the probiotic strains or to toxin adsorption by
bacterial cells. Immunological assays showed that
there was significant diminution of both clostridial
toxins (TcdA and TcdB) in spent culture supernatants
of co-cultures as compared with pure clostridial
cultures. Even though co-cultured clostridial cells
showed a slight increase of intracellular toxins, this
increase did not completely explains the reduction of
toxin concentration in culture supernatants. The
evidence suggests that the antagonism could be due
to the diminution of the synthesis and/or secretion of
both clostridial toxins. Our findings provide new
insights into the possible mechanisms involved in the
protective effect of probiotics in the context of C.
difficile infection.
difficile infection.
difficile infection.
C.
difficile infection.infection.