INVESTIGADORES
PEREZ Pablo Fernando
artículos
Título:
Protective effect of bifidobacteria in an experimental model of Clostridium difficile associated colitis.
Autor/es:
TREJO, F. M,; DE ANTONI, G. L. AND PÉREZ, P. F.
Revista:
Journal of Dairy Research
Editorial:
CAMBRIDGE UNIV PRESS
Referencias:
Lugar: Cambridge; Año: 2013 vol. 80 p. 263 - 269
ISSN:
0022-0299
Resumen:
The aim of this study was to evaluate the ability of Bifidobacterium strains to prevent the effects
associated with Clostridium difficile infection in a hamster model of enterocolitis. After clindamycin
treatment (30 mg/kg), animals were infected intragastrically with C. difficile (5×108 CFU per animal).
Seven days prior to antibiotic administration, probiotic treatment was started by administering
bacterial suspensions of bifidobacteria in drinking water. Strains CIDCA 531, CIDCA 5310, CIDCA
5316, CIDCA 5320, CIDCA 5323 and CIDCA 5325 were used. Treatment was continued during
all the experimental period. Development of diarrhoea, enterocolitis and mortality were evaluated.
All the infected animals belonging to the placebo group developed enterocolitis (5/5) and only two
dead (2/5) whereas in the group administered with Bifidobacterium bifidum strain CIDCA 5310 the
ratio of animals with enterocolitis or dead decreased significantly (1/5 and 0/5 respectively).
Biological activity of caecum contents was evaluated in vitro on Vero cells. Animals treated with
strain CIDCA 5310 presented lower biological activity than those belonging to the placebo group.
The present study shows the potential of selected strains of bifidobacteria to antagonise, in vivo, the
virulence of C. difficile.
Bifidobacterium strains to prevent the effects
associated with Clostridium difficile infection in a hamster model of enterocolitis. After clindamycin
treatment (30 mg/kg), animals were infected intragastrically with C. difficile (5×108 CFU per animal).
Seven days prior to antibiotic administration, probiotic treatment was started by administering
bacterial suspensions of bifidobacteria in drinking water. Strains CIDCA 531, CIDCA 5310, CIDCA
5316, CIDCA 5320, CIDCA 5323 and CIDCA 5325 were used. Treatment was continued during
all the experimental period. Development of diarrhoea, enterocolitis and mortality were evaluated.
All the infected animals belonging to the placebo group developed enterocolitis (5/5) and only two
dead (2/5) whereas in the group administered with Bifidobacterium bifidum strain CIDCA 5310 the
ratio of animals with enterocolitis or dead decreased significantly (1/5 and 0/5 respectively).
Biological activity of caecum contents was evaluated in vitro on Vero cells. Animals treated with
strain CIDCA 5310 presented lower biological activity than those belonging to the placebo group.
The present study shows the potential of selected strains of bifidobacteria to antagonise, in vivo, the
virulence of C. difficile.
Clostridium difficile infection in a hamster model of enterocolitis. After clindamycin
treatment (30 mg/kg), animals were infected intragastrically with C. difficile (5×108 CFU per animal).
Seven days prior to antibiotic administration, probiotic treatment was started by administering
bacterial suspensions of bifidobacteria in drinking water. Strains CIDCA 531, CIDCA 5310, CIDCA
5316, CIDCA 5320, CIDCA 5323 and CIDCA 5325 were used. Treatment was continued during
all the experimental period. Development of diarrhoea, enterocolitis and mortality were evaluated.
All the infected animals belonging to the placebo group developed enterocolitis (5/5) and only two
dead (2/5) whereas in the group administered with Bifidobacterium bifidum strain CIDCA 5310 the
ratio of animals with enterocolitis or dead decreased significantly (1/5 and 0/5 respectively).
Biological activity of caecum contents was evaluated in vitro on Vero cells. Animals treated with
strain CIDCA 5310 presented lower biological activity than those belonging to the placebo group.
The present study shows the potential of selected strains of bifidobacteria to antagonise, in vivo, the
virulence of C. difficile.
C. difficile (5×108 CFU per animal).
Seven days prior to antibiotic administration, probiotic treatment was started by administering
bacterial suspensions of bifidobacteria in drinking water. Strains CIDCA 531, CIDCA 5310, CIDCA
5316, CIDCA 5320, CIDCA 5323 and CIDCA 5325 were used. Treatment was continued during
all the experimental period. Development of diarrhoea, enterocolitis and mortality were evaluated.
All the infected animals belonging to the placebo group developed enterocolitis (5/5) and only two
dead (2/5) whereas in the group administered with Bifidobacterium bifidum strain CIDCA 5310 the
ratio of animals with enterocolitis or dead decreased significantly (1/5 and 0/5 respectively).
Biological activity of caecum contents was evaluated in vitro on Vero cells. Animals treated with
strain CIDCA 5310 presented lower biological activity than those belonging to the placebo group.
The present study shows the potential of selected strains of bifidobacteria to antagonise, in vivo, the
virulence of C. difficile.
Bifidobacterium bifidum strain CIDCA 5310 the
ratio of animals with enterocolitis or dead decreased significantly (1/5 and 0/5 respectively).
Biological activity of caecum contents was evaluated in vitro on Vero cells. Animals treated with
strain CIDCA 5310 presented lower biological activity than those belonging to the placebo group.
The present study shows the potential of selected strains of bifidobacteria to antagonise, in vivo, the
virulence of C. difficile.C. difficile.