Penicillium corynophilum lipase production using an airlift agitated fermentor

RODRIGUEZ, EMILIO; PERA, LICIA MARÍA; BAIGORI, MARIO DOMINGO

Tucumán

Jornada; XX Annual Scientific Meeting; 2004

Asociación de Biología de Tucumán

Penicillium corynophilum lipase production using an airlift agitated fermentor   Rodríguez, Emilio; Pera, Licia M. and Baigorí, Mario D.   PROIMI Av. Belgrano y Pje. Caseros, 4000 Tucumán. Tel: 4344888, e-mail: mario@proimi.edu.ar     Introduction. Lipases (triacylglyverol hydrolases, EC 3.1.1.3) are ubiquitous enzymes acting on lipid substrates at the lipid-water interface. They are mainly used in synthetic organic chemistry, catalyzing the hydrolysis of carboxylic acid esters in aqueous medium or the reverse reaction in organic solvents. Materials and methods. Pellets formation was performed at 30°C, on an orbital shaker using M1 medium. Comparative production experiments took place in an airlift agitated fermentor using M1 or M2 media. Media: M1 (g/l): glucose 10; NH4NO3 2; KH2PO4 1; MgSO4.7H2O 1,1; ZnSO4.7H2O 0,15; FeSO4 0,01; pH7; 2% olive oil. M2 (g/l): glucose 2; NH4NO3 0,5; KH2PO4 0,2; MgSO4.7H2O 0,2; FeSO4 0,001; pH4,5; 2% olive oil. Lipase measurement was carried out with standard techniques. Protein and lipase zymograms were performed with Coomassie blue and a-naphthyl acetate/fast blue salt B, respectively. Results and conclusions. Both lipase specific activity and fungal morphology was strongly improved when M2 medium was used. According to PAGE assays only one activity band was observed. This work was supported by grant CABBIO 2000 cod 012