Cholesterol characterization of human follicular fluids from patients at risk of developing ovarian hyperstimulation syndrome (OHSS

PASCUALI N, QUIROZ A, SCOTTI L, DI PIETRO M, OUBIÑA G, DE ZÚÑIGA I, ABRAMOVICH D, BUSSO D, PARBORELL F; DOLORES BUSSO

Buenos Aires

Congreso; XVIII Jornada Anual de la Sociedad Argentina de Biología (SAB); 2016

Sociedad Argentina de Biología (SAB)

CHOLESTEROL CHARACTERIZATION OF HUMAN FOLLICULAR FLUIDS FROM PATIENTS AT RISK OF DEVELOPING OVARIAN HYPERSTIMULATION SYNDROME (OHSS)Pascuali N1, Quiroz A2, Scotti L1, Di Pietro M1, Oubiña G1, de Zúñiga I3, Abramovich D1, Busso D2, Parborell F1.1Instituto de Biología y Medicina Experimental (Buenos Aires, Argentina) 2Depto. de Nutrición, Diabetes y Metabolismo, Fac. de Medicina, Pontificia Universidad Católica de Chile (Santiago, Chile) 3 Centro Médico Pregna (Buenos Aires, Argentina) - natypascuali@gmail.comOHSS is a complication of ovarian stimulation with gonadotropins following human chorionic gonadotropin (hCG) administration. Enlarged ovaries, increased vascular permeability and excessive production of steroid hormones and vasoactive compounds are main features of this syndrome, though its exact pathophysiology remains unknown. The aim of this study was to characterize the cholesterol and lipoprotein profile of human follicular fluids (hFFs) from patients at risk of developing OHSS. For hFF collection, aspirates were obtained from patients 25?39 years old undergoing oocyte retrieval at the Reproductive Medicine Center Pregna and were classified into control group or OHSS group. Cholesterol concentration was obtained by an enzymatic assay, apolipoprotein A-I (ApoAI) levels were measured by western blot and lipoprotein cholesterol profiles were assessed by fast-performance liquid chromatography (FPLC). Total concentration of cholesterol was higher in OHSS hFF than in control hFF, while ApoAI levels were significantly lower in the OHSS group. Finally, VDLD, LDL and HDL elution fractions from FPLC chromatograms were analysed. The HDL-cholesterol peak was significantly higher and also slightly shifted to the left in OHSS hFF compared to control, indicative of larger particles containing more cholesterol. In conclusion, cholesterol metabolism seems to be altered in OHSS, providing useful information for novel approaches to this pathology